FastQCFastQC Report
Mon 11 Jul 2016
MG401672S_S15_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401672S_S15_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959921
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG3957.658127E-826.159871
TAGACTG4402.1772757E-723.4807595
ACTGTGC4653.7097925E-722.2183518
CTTAGGC2650.002824632721.6593993
TAGCCTG3253.697509E-421.192895
TAGCCCT4405.703405E-620.8717864
GTATAGG6651.2641976E-920.7180921
CCGCTCA4006.473996E-520.0890929
CCAGGAC2950.005256982519.4567493
GACCGTG4209.4187024E-519.132477
ACCGCTC4251.0314172E-418.9073838
ACTACAC6253.099667E-718.3671723
CTGTGCG3900.001261140917.6607429
GTGTGGG4601.8886566E-417.4715081
TAATACA17900.017.315424
ATTAGAC9503.3651304E-1016.9171313
TAGGACT6807.503113E-716.8815924
GTATACG10356.366463E-1116.6395321
CTTATAG7101.1779557E-616.1682853
GTATAGA8703.5272024E-815.8362431