FastQCFastQC Report
Mon 11 Jul 2016
MG401672S_S15_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401672S_S15_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959921
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG1200.001564016838.252228
TAGAGTG4355.456968E-1231.6570115
CCGTGAG2557.1543596E-527.001579
GTACTAC3851.7952243E-623.8455411
ACAGGGC2951.9246315E-423.3403388
TACCGTG4704.1254862E-721.9746827
GTATATG12250.021.5461521
TTATACT13700.020.103364
CAGGGCA3506.108516E-419.6725739
TAGCCTA4751.1083606E-519.3274385
TACTGAG5401.5647529E-619.126117
GTCTAGA5652.412282E-618.2798231
TCTACAC10101.8189894E-1218.1792743
TATAGGG4451.4710063E-418.051613
GAGAGTC3250.00920997717.6548717
ACCGTGC3900.00126395517.6548718
GTCCTAT5252.6237305E-517.486731
GTACCAC4601.8957279E-417.462976
CCATGCG5403.341525E-517.0009889
TATACCG6756.9698217E-717.0009885