FastQCFastQC Report
Mon 11 Jul 2016
MG401669P_S17_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401669P_S17_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700375
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGC1350.00280076233.9689528
ACAGGGC2801.3594021E-424.5668328
GTATAGG5251.895387E-924.0243321
GTCTAGG3002.1680433E-422.9323161
CTGTGCC4002.5223708E-622.9290439
ACTGTGC3552.6046442E-522.6060988
TAGAGAG4203.852305E-621.8371835
CCTAGAC3253.728826E-421.165272
CCTTAGG2850.00433773920.1131952
TATACTG9700.020.092465
GTGTAGG5401.5759761E-619.1102641
CCCTTAT7354.458343E-918.7202591
TACTGTG5552.051127E-618.5911167
AGCGTGC3150.007733337618.1976558
TAGACTG3900.001271749317.6377265
TCCTAGG3250.00925922817.6377262
ATACGGG3900.001271749317.6377263
ATTATAC14450.017.4546363
GTAGGAC3950.001385098317.4144613
GTATATT13300.017.2423441