FastQCFastQC Report
Mon 11 Jul 2016
MG401669P_S17_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401669P_S17_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700375
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGG700.00907713849.072321
TGGGGCC700.00907713849.072324
GGAGTGC2253.0930678E-530.5338868
TATACAG10500.027.2623985
GTGTGTG2209.7030704E-426.02329
TATAGAC5358.54925E-1125.6827073
ACAGGGC3151.0423937E-525.4449048
TAGAGAG4351.9968866E-723.6900845
CATATAC10000.022.9004143
CAGGGCA2550.002292360922.4513859
TACAGGG4603.423338E-722.4025787
GTATACG7359.094947E-1221.809921
CTTAGGC3153.044603E-421.8099173
GTATAGG4456.4257456E-620.584641
ATACAGG9750.019.9644646
TATAGGA6351.7289494E-819.8350034
CTATAGG2900.00483175919.7417351
CTATACA7603.237801E-1019.5858784
GTACTAG4107.978514E-519.5491331
GTTAGAC4158.757052E-519.3136023