FastQCFastQC Report
Mon 11 Jul 2016
MG401595Q_S18_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401595Q_S18_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722087
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACCCT2107.4180786E-427.247894
ACTGTGC3805.4260454E-827.104488
GCCTATA3251.3308778E-524.655691
TATACTG7800.023.4751075
TATACCG5305.6270437E-821.5926695
GTATATA9700.021.2423841
GTACTAT5508.299321E-820.8132461
CTGTCCC4659.440373E-619.6887979
AGACTGT7153.2414391E-919.2069036
ACCGCAC3000.00589601319.0735238
AGGGTTA6102.478355E-718.7608436
GGACAGG4301.154739E-418.6299536
AGGGTGA3100.0071246818.4582486
AAGGGTG4351.2617455E-418.4158155
CTGGCCA3150.00781341518.165269
GTATAGG5051.9182271E-518.1343121
GTATAGA6353.7681093E-718.027221
GTACACG3900.001284693117.6112081
ACTATAC5954.0544546E-617.3104233
GTCCTAT4702.2760133E-417.0491471