FastQCFastQC Report
Mon 11 Jul 2016
MG401595Q_S18_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401595Q_S18_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722087
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC1954.8232605E-429.328936
ACTGTCC5256.91216E-1126.144658
ATACTGT10150.024.792346
TACTGTC6950.024.6869417
GTATATA8100.024.0062751
TATAGTG7250.023.6654135
TATACTG8350.023.2875235
TATACAG10100.022.6500665
GCACCGT3052.4651343E-422.5015416
CTTAGAC4805.209622E-721.4467833
CATATAC8101.8189894E-1221.1820073
ACTGTGC3253.7871252E-421.116838
CTAGGAC3304.1982677E-420.7968793
CCTATAC5058.486204E-720.385063
CTATACA6851.9153958E-920.037874
TTAGACT5801.4654324E-719.7211784
CCTGTGC2950.00536611919.386928
TTACCCC2950.00536611919.386924
ACCCTGT3709.0710924E-418.5485676
GCTATAC6803.8382495E-818.5031053