FastQCFastQC Report
Mon 11 Jul 2016
MG401586Y_S16_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401586Y_S16_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622051
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGCG1400.003281752632.887395
ACCGTGC2854.5973975E-628.2716148
CTAGGAC2455.333577E-528.189193
CAGGGCT2158.2267635E-426.7688059
TATAGTG5451.0186341E-1025.3444085
GTCTATA5151.4479156E-924.5876941
GTACTAC3401.8069106E-523.7001691
CACCGTG2450.001761701523.4909927
GTACTGC5002.8512659E-823.0230221
TATACCG4153.3524993E-622.1890815
CTATAAG5755.180482E-922.0220221
TACACCG5906.9794623E-921.4604155
GTATAAT12400.021.3521
ACAGTGC3404.920115E-420.31288
TACCGAG3404.920115E-420.31287
CCTTAGG2850.00423769120.194012
CCGTGCA2850.00423769120.194019
TCTAGAC4006.336052E-520.1435243
GCACTGA5159.637897E-720.1155876
TAACACT6401.780063E-819.783824