FastQCFastQC Report
Mon 11 Jul 2016
MG401586Y_S16_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG401586Y_S16_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622051
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAC2104.258909E-738.3380473
CGTAGGG1500.00461829830.670443
GGAGTGC2608.036935E-526.5417278
CTAGACT3108.891573E-625.9709384
GTATACT6200.025.9709384
CTAGGAC3151.0066942E-525.5586993
GTAGACT3751.3988192E-624.536354
GTATAGG4002.4522033E-623.002831
CGTATAC5553.4779077E-922.7955973
TAGACTC2550.002233412322.5517925
TATACTA6154.656613E-1022.4417845
CCTATAC4103.0386927E-622.4417843
CTAGACC4203.7453374E-621.9074554
TAAGCCT5457.165545E-821.1035124
GTCTTAT7701.6370905E-1120.9116631
TAGACTG2800.003842576420.538245
TATACTC5108.843872E-720.2966125
TCTATAG4057.014089E-519.8789883
CCTACAG4709.913088E-619.5768763
TCCTATA4709.913088E-619.5768762