FastQCFastQC Report
Mon 11 Jul 2016
MG400228D_S13_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG400228D_S13_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025168
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACT6900.024.9010964
TATACAG12300.022.350255
GTATAGG7153.2050593E-919.2271561
GGTTAGG3100.007083473718.477711
GTGTATA10750.018.116751
TAGACAG9001.6007107E-1017.8181175
CCTATAC6454.4144508E-717.758923
GTCCTAT4652.0861204E-417.2458631
TATAGGA9352.764864E-1017.1511292
TAGAGTG4702.2662489E-417.05995
TATACTG12100.017.0397595
GGAGTGT4752.456954E-416.8803226
CTATACC8201.751323E-816.762694
GTATATG14500.016.591711
GTGTGCA4200.002101485216.3635799
ATTAGAC11301.4551915E-1116.2187673
CCCTATA4953.3612468E-416.200661
GTGTATG7851.9437175E-716.0532471
GTATAAG10751.1641532E-1015.9853681
GTATTAT21650.015.8746151