FastQCFastQC Report
Mon 11 Jul 2016
MG400228D_S13_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG400228D_S13_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025168
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4458.749339E-925.7348163
TAGGACC4301.7849197E-723.9692864
TATACTG12050.023.759325
AGAGTGT4553.079349E-722.6522926
TCTATAC10700.020.3353123
CTAGAGC5301.3345816E-619.446783
GGACTGT7804.5838533E-1019.0866556
TAGGACT5602.2603072E-618.4049874
CCAGGGA3850.001175424917.847264
GGGACTG7106.2122126E-817.7425235
CCCTTAG3250.00932057317.618451
GTATACA11700.017.618451
GGTTGCG3900.00128165617.618458
CCTAGAC4551.7716366E-417.6184483
GTAGGAC5252.670975E-517.4506553
CTATACA9953.6379788E-1117.264314
ATACTGC12250.016.8274176
ACCGTCG4100.001791450216.7590128
TACACTG8201.7560524E-816.7590125
CTAATAC22950.016.4669173