FastQCFastQC Report
Mon 11 Jul 2016
MG400034J_S14_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG400034J_S14_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291695
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC3052.3899341E-422.6067545
TAGACCG6357.039489E-1021.7167245
TCCAGGG4355.120688E-621.1342812
GTATACG12150.020.8113571
GTATAGA14850.020.1233791
CATATAC17400.019.8133893
GTCTTAT15900.018.7944771
GTATATA15450.018.5979711
GTCCTGG4451.4540674E-418.0797351
GACCGTC5102.021406E-518.02637
TGAGACC4501.5857982E-417.876084
TATACAG18750.017.7739325
ACCGTCC4752.397395E-416.9352368
GTGTAGA13950.016.4782031
ACAGGGC4200.00205676516.416818
TAGGACT9103.414243E-916.416814
CTCATAC10608.913048E-1116.2619343
TAGACCC4953.2835844E-416.2509825
GTACTAG8603.0224328E-816.0375061
TTATACC18000.015.9607864