FastQCFastQC Report
Mon 11 Jul 2016
MG400034J_S14_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG400034J_S14_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291695
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG3507.735998E-726.2657181
CTAGGAC5802.2737368E-1023.7750033
GTATAGG9450.021.8880961
TAGGACT9550.020.4556314
TATACTG16450.020.2581335
TATACCG9800.019.9338045
TAGTCCG4701.0002694E-519.5595769
GCCCTAC3050.0063370518.8381181
ACACTGC7055.513357E-817.9296136
TCTATAC14100.017.9296133
TACCGCG3850.001148968417.9084457
CTACACT11000.017.7592074
CCTACAC11300.017.2877243
GGACTGA13300.017.2800796
ACAGGGC4050.001613201117.0240768
CCCTACA7451.0354597E-716.9669482
TAGGACC5453.5760862E-516.8678934
CTATACT14100.016.2996484
ACTCTAT10701.03682396E-1016.109231
GTCTAAG7151.2547844E-616.071681