FastQCFastQC Report
Mon 11 Jul 2016
MG399601N_S19_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG399601N_S19_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976181
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCT4852.1664164E-823.6302725
CAGGACC4553.0565934E-722.6694834
CTATACT9700.022.448764
TAGGACC4203.866089E-621.8298724
AGGGGGC2900.004807599419.7597988
GTGTAGG5951.8761057E-719.26561
GAGTTCC4251.04449166E-418.876429
TATAGGA8557.6397555E-1118.766032
GTATAAA19100.018.604921
ATCTAGG6854.076537E-818.4077871
GTCTACG5001.7410943E-518.340851
GTATAGG6904.4305125E-818.2743971
TATACTG12550.018.2640365
GTGTAAG5702.6519392E-618.0995241
GGACCTA3900.001275095417.631826
TCTAGAC5853.4047043E-617.6318193
CTATAGG4652.0764841E-417.2561761
GTGTATA10705.456968E-1217.1409821
CTATAGC6757.050912E-716.982271
TCCTAGG5453.6572288E-516.823022