FastQCFastQC Report
Mon 11 Jul 2016
MG399601N_S19_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG399601N_S19_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976181
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGG1650.007508542427.7680931
CTAGGAC3906.924893E-826.4330883
TAGACAG7700.025.28885
AGAGGCC2751.2106354E-424.9912838
CCTAGGA4751.7467755E-824.1143972
TAGGCTG6252.3646862E-1123.8250265
ACTGTGC5305.5793862E-821.611968
CTATACA9800.021.038584
GTATAAT17000.020.213541
ACCGTCG3405.0881755E-420.2135378
GACTGAG5251.2161854E-619.636017
TCTATAC8204.1836756E-1119.5561893
TATATAC14750.019.4141333
ACAGACC4751.126327E-519.2915178
TATACAG15850.018.789455
ACAGGGC3050.006454759318.7776038
CTTAGGC4451.492141E-418.018069
GCCTACA5752.9035145E-617.928531
ACCGTGC3200.00851396317.8974048
TCTAGGG4501.6253731E-417.817862