FastQCFastQC Report
Mon 11 Jul 2016
MG398768Y_S4_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG398768Y_S4_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985301
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGTA4255.180482E-927.0323669
TGGGCCG2158.32195E-426.7180355
TAGAGTG3351.6372749E-524.0063535
CACCGTG4653.6812526E-722.2363017
GTGTAGA11450.022.0789451
TAGACTG5805.86806E-921.7890175
TAGGCAC4254.1939893E-621.6258935
CTAGGCA3253.6774974E-421.2100094
GTATAGG5507.978633E-820.8928851
TACACTG8900.020.6539425
CTAGACT5751.2781493E-719.9804424
CTATACC6901.9863364E-919.9804424
GTATAAG12100.019.9432091
ACTATAC7703.6925485E-1019.3965993
CCTAGGA3607.3276146E-419.1479242
AGGGAGC4209.3608214E-519.1479246
GTCTAGG3759.627962E-418.385741
TAGACAG8157.894414E-1018.325625
TATAGGA8901.3096724E-1018.0721992
ATAGGAC5152.2024344E-517.846613