FastQCFastQC Report
Mon 11 Jul 2016
MG398768Y_S4_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG398768Y_S4_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences985301
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAT1853.4562362E-431.047131
ACACTGC4052.7633323E-622.6912366
TATACTG11450.022.0719325
AGGGTAG2650.002813350921.6744125
GTATAGA9550.021.6517161
CCGTGCA4855.527327E-721.3168959
CTATCCT7101.2551027E-1021.0333374
GAGAGCG2800.003871314720.5132836
ACCGCAC2850.00428910820.15348
AGTGTGC3455.5062043E-419.9781518
TATATAC11850.019.388083
GTGTGCG3150.00764695918.2340289
CACCGCG3150.00764695918.2340289
GTGTAAG6954.6966306E-818.1815571
TAGGACG5702.5986665E-618.138064
GACCGTG4451.4595539E-418.0701267
GTATAAT17400.017.8253351
GTACCCT3250.00915592917.6729814
ATACTGT13900.017.3551226
TTATACT12850.016.9853174