FastQCFastQC Report
Mon 11 Jul 2016
MG398073N_S5_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG398073N_S5_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896158
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGTC1651.8255143E-434.613517
ACCGTGC3754.8628863E-827.4138988
TCCTAGG3703.689727E-521.6100542
GTCTTAT10800.021.156241
ACAGACC6009.26957E-920.9411728
GTATAGA8850.020.6542281
AGGGTGT3506.302263E-419.5813546
TATACTG11350.019.1212945
TAGGGAG3658.3580305E-418.7766425
CACCGTG3750.001002218618.2759327
GTATAAG9552.0008883E-1117.9440351
TAAGACC5102.1281881E-517.917584
TATAGGA7708.3273335E-917.8012312
GTCCTAC4501.6580943E-417.771241
AGGGAGT5152.3144114E-517.7436226
TATATAC12100.016.9920853
GTCTTAG6105.2273735E-616.8556271
ATTATAC16400.016.7157923
CAGTGCA6858.5318607E-716.675129
GTATAAT15800.016.6304111