FastQCFastQC Report
Mon 11 Jul 2016
MG398073N_S5_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG398073N_S5_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896158
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC3508.1773396E-726.0975258
ACCGGGT2209.867722E-425.9492448
TAGACTG4553.168825E-722.5843965
GTCTAAG4704.3310683E-721.8636171
ACCGTCC3153.1046805E-421.7479388
TATACTG10550.021.6448675
TCTATAC9000.021.5667063
AGGACCG2650.002914379121.5427685
CCCTTAG2700.0032481121.1438271
TACCGCG3304.2507937E-420.7593967
CTCACAC2800.00401006320.388693
TCTAGGG2850.0044427320.0309962
TAGACAG7502.8194336E-1019.7906235
CACCGTA6952.3483153E-919.7139597
GTCTAGG3506.3200865E-419.5731431
GTATAAG11150.019.4561141
GTATAGC8254.9112714E-1119.3754351
CTATACT9651.8189894E-1218.9308474
ACAGTGC4851.38560645E-518.8332658
ACACTGC4951.6519738E-518.4527956