FastQCFastQC Report
Mon 11 Jul 2016
MG397296P_S20_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG397296P_S20_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647609
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAC1450.00400254431.5807883
TATACTG8350.024.6783985
TACAGTG5302.1536835E-923.7600757
ATAGGAC3754.0199368E-521.3696653
CTATAAG5457.521703E-821.007191
GTATAGC6551.0822987E-920.9751191
TAGCCCC2750.003556383820.814614
TATACCG5058.4142994E-720.4024375
TATAAGA12250.019.6252022
GTGTATA5851.5878686E-719.57081
TTATACC8204.1836756E-1119.5454254
CACTGTG2950.00533916919.403459
GTATTAG10700.019.2598651
TAACACT5951.8996798E-719.2403954
GTGTAAG4209.607279E-519.0815321
TATACAG9651.8189894E-1218.9811975
TATACCC3658.229935E-418.8186875
CTATACT7404.944013E-918.564384
TATAGAG6253.1862874E-718.3168562
AGGACAG5001.7593391E-518.3168545