FastQCFastQC Report
Mon 11 Jul 2016
MG397296P_S20_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG397296P_S20_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647609
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGT2253.1153017E-530.501364
CTAGGGG2056.462868E-427.8975854
CCTAGAC2506.3903615E-527.4512233
CCTATAG2209.7628176E-425.9954761
CCCTATA2659.499514E-525.897382
GTCTTAG4852.2095264E-823.5835231
GTCTAGA3502.3760147E-522.876021
TACACGG2550.002306414822.427475
ACAGGGC3102.7518373E-422.1380848
GGACCTA2600.002581763321.9961726
GTACTAG3304.1983023E-420.796381
CCTAGAG3354.6466102E-420.4859873
CAGGGCA2850.004395902620.0666839
GTCCTAT3506.242221E-419.6080171
CATATAC9601.8189894E-1219.063353
TTAACCC3607.5452635E-419.063353
TACTGGG4851.3635547E-518.866822
GTCTAAG3658.27855E-418.8022081
ACAGTGC3100.00714586718.4484048
GTATAAA15700.018.2133921