FastQCFastQC Report
Mon 11 Jul 2016
MG396838G-2_S7_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396838G-2_S7_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933867
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG3057.662324E-626.4758647
TACACTG7205.456968E-1222.430945
TACACCG9600.021.6298355
TAGGACC3753.7920574E-521.5337034
ACTATAC7651.4551915E-1121.1114733
GTATAGG6601.0822987E-920.9777571
CTAGTAC7152.9394869E-919.3609713
TAGACAG8455.820766E-1119.1127535
TGGGGTG4259.932923E-519.0003265
GGAGTCC3050.006195437618.9113316
GTATAGA10151.8189894E-1218.187611
CCGTGCA5101.9548725E-518.0955499
CTAGGCA4501.5393563E-417.9447544
CTGTACC11000.017.8282284
GTATTAG18200.017.7504081
TAGACTG5853.1983946E-617.7475595
TATACAG14400.017.6243115
GTATAGC9201.9826984E-1017.557471
CACCGTG4651.9781895E-417.365897
TAAACCT12100.017.1608624