FastQCFastQC Report
Mon 11 Jul 2016
MG396838G-2_S7_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396838G-2_S7_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences933867
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGTC2556.9139816E-527.137374
CTTACAC6800.025.4412843
GTACACT6051.4551915E-1124.7824734
TACACTG8700.022.5364955
TAAGGGT4102.9689927E-622.504164
CTAGGAC4102.9689927E-622.504163
GTATATA11800.021.5029161
CTCTTAG5406.320988E-821.3581161
GTATAGG5957.532435E-921.322221
GCCCTAA3404.8574174E-420.3530271
CCTATAC6052.0961306E-719.063443
TCCTATG6102.2856875E-718.9071852
TATATAC12900.018.7752743
GTGTAGG6252.950619E-718.4534111
AACCGTG6303.2082062E-718.3069557
TAACCCG3150.007472663718.3069555
GTGTATA10251.8189894E-1218.0033281
CTTAGAC5152.1300257E-517.9159343
GGAGTGT5252.512929E-517.5746786
GTGAGTG4601.8243173E-417.5507989