FastQCFastQC Report
Mon 11 Jul 2016
MG396838-1_S6_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396838-1_S6_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855643
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGC3251.2599916E-524.8313523
GCCGTGC3102.6094096E-422.3138878
GTGTATG7307.2759576E-1222.112691
GTATATA9650.021.5070881
TACCGTG4354.976562E-621.2024677
CCGTACG2750.003415204120.9615299
TGTGTGC3854.6663277E-520.9615298
GTGTAAG5558.4661224E-820.7751161
GGACCGC2800.003791070820.5872176
AGGCCGT2900.00464502919.8773126
ACCGTGC3505.9205346E-419.7637278
TATATAG9400.019.623565
TATACCG6102.2946188E-718.899745
GTATATG9251.0913936E-1118.6976031
ATAGTGG5551.9452982E-618.6954178
GTCTTAA6803.504283E-818.6517751
TAGACTA6202.7224087E-718.5949065
TAAGGGG3850.001123647617.9670264
AAGGGGG5252.521048E-517.5677575
GTCTACG4601.8279583E-417.545941