FastQCFastQC Report
Mon 11 Jul 2016
MG396838-1_S6_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396838-1_S6_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences855643
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGGC1450.00386794831.8033923
GTATAGG6000.028.8218271
GGACCGT3351.593513E-524.0898846
CTAGGAC3901.929042E-623.6486783
GTCCTGG3502.2363405E-523.057461
TAGGGGC3753.8098333E-521.5202985
TATAGGG4304.5026372E-621.44882
GACCGTC4456.05935E-620.7258057
CCAGGAC2800.003791283120.5870173
ACAGTCC6251.3327735E-820.2905648
GCAGTGC4709.719104E-619.623378
TATACAC9109.094947E-1219.00343
CTTACAC6102.2948552E-718.8995593
TAGGACC3100.006829201718.5947274
GACCGTG4351.1928021E-418.551987
GTAGGCT4351.1928021E-418.551984
TAGGCTG6353.4997902E-718.155485
TATATAC9551.8189894E-1118.1079523
CTGCCCT3850.001123719817.9668529
GTAGTAC3300.00979175117.4677733