FastQCFastQC Report
Mon 11 Jul 2016
MG396050R_S8_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396050R_S8_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971705
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCC2659.029635E-526.0932738
ACTACGC3609.640517E-725.6100658
GTATAAG10200.024.8645061
CTTAAGG2400.001549339424.0168511
GTTAGTC5355.776201E-821.5411763
ACCGTAC4304.517924E-621.4409838
TATACTG14150.021.1758145
GTATATG12200.020.7883551
ACAGTGC3955.702433E-520.4232168
GTGTAGG7502.4920155E-1019.982021
GTACTAC4056.8953595E-519.925091
GTAGGGG2950.005139335519.53313
GCAGGGC2950.005139335519.53318
CTAGAGT4209.139877E-519.2075484
CTACACC10850.019.1190364
CTAAGGG3657.8734424E-418.9444313
GTATAGC10350.018.9350241
CCGTACC3708.627517E-418.6884279
GTGTATA10051.8189894E-1218.3531761
GTCCTAG3850.00112428817.9658531