FastQCFastQC Report
Mon 11 Jul 2016
MG396050R_S8_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG396050R_S8_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971705
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC6150.029.9793033
CTAGGCA3102.6182752E-422.303154
TACACCG9350.022.1838825
CTATACT11700.021.6677324
TCTATAC11900.021.3035683
TCTAGGC4405.5185064E-620.9514433
CTATACA11600.020.8611354
GTGTAGG7351.9099389E-1020.3813361
CCTATAG4056.917455E-519.9168031
CACCGTC5351.3757526E-619.3849817
CCCTATT6102.3070606E-718.8906462
AGGCGTC3100.006848464718.5859587
TAGGGGT3100.006848464718.5859584
ATAACAC10550.018.568343
TACTCCG4351.1973349E-418.543235
GCTCTAG3800.001032739218.1946741
ACCCTAT3850.001127430617.958381
ACAGACC5152.1462607E-517.9002638
TGTAGGG4601.8366627E-417.5354482
GAGTAGG4651.994912E-417.3468931