FastQCFastQC Report
Mon 11 Jul 2016
MG395417Q_S10_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395417Q_S10_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927312
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGG1101.6855343E-551.8393361
ATAGGGC2251.7113052E-840.5433243
GTATATA12500.023.7216821
TATACAG12150.023.4625725
AGCGTGC4052.9462535E-622.5240678
CAGGGCT3052.5182488E-422.4317574
ACAGGGC2550.002348843722.358458
AGACCGC4103.277346E-622.2493846
GAGGGCA3153.1322413E-421.7196379
CCTAGGA3703.739228E-521.5728842
AGACTCG2650.002936556721.5147345
ACCGTGC3253.8685362E-421.051348
GTATAGG5701.2594137E-720.0081631
TAGACTA5751.3832141E-719.830975
CAGGTCA5301.3904682E-619.363269
CCTACAC5951.9827894E-719.1643853
TCCTAGG3607.706741E-419.0046832
TATAGGA8406.366463E-1119.004682
CCCTAAC3709.2550094E-418.4940341
CTGTGCG3100.00727636918.3916289