FastQCFastQC Report
Mon 11 Jul 2016
MG395417Q_S10_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395417Q_S10_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927312
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGCG2905.6717145E-627.5209399
GTCTAGG3559.369851E-725.693591
ACCGTAC4456.6708144E-620.4971358
TAGACTG4659.754112E-619.6155385
CCTATAC6401.988883E-819.5963823
TACACTG7203.7071004E-919.002555
ACAGACC4901.5322974E-518.6147448
GTCTTAG5552.1636042E-618.488971
CTAGACC6253.329278E-718.2424494
GTATATA12500.018.2424491
GTGTAGG6404.2700594E-717.8148921
ATCTAGG7809.995347E-917.5408171
GTATAAA20350.017.3684251
TATACAG13150.017.3407335
GTATATG11950.017.1738551
TATATAC12300.016.6851673
CTTATAC10458.185452E-1116.3658333
TCTACAC9204.3764885E-916.1108593
TAGTACT5705.6125285E-516.002154
ACACTGC4300.002536018615.9091146