FastQCFastQC Report
Mon 11 Jul 2016
MG395416N_S9_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395416N_S9_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205594
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCC2005.485563E-428.6931137
CCTGGGC2056.337942E-427.993288
GTATAAG13050.021.987971
TATACAG17550.021.5811425
GTGTAAG7852.1827873E-1120.469821
GTCCTAG5751.2917735E-719.9612541
GTATAGA12850.018.7573641
GCACCGT6152.6232192E-718.6621866
AGGGACC3100.00700949318.5116845
TATATAC16150.018.4772993
TGGGGAG10001.8189894E-1218.3635925
GTCTAAG7106.0574166E-817.7823851
CCTATTC9151.9826984E-1017.560813
GTATATA16400.017.4965271
TGGACCG5953.9487077E-617.3605385
ACTGTCC10605.456968E-1217.3241448
TATACTG14700.017.1768285
GTATACA10857.2759576E-1216.925671
GTCCTAT5554.2212043E-516.5444621
TATAAGG13900.016.514022