FastQCFastQC Report
Mon 11 Jul 2016
MG395416N_S9_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395416N_S9_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205594
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGGC1750.00991784326.2284098
GTCCTAT5902.8558134E-1023.3388391
TATACCC5609.7996235E-820.4909445
ACTGTGC6751.5352271E-920.3998748
TAGTGCG3955.8964055E-520.3353189
GTATAGA12550.020.1154121
GGAGTCC3050.006389032618.8113578
CTATACT12300.018.6584194
GTATAGG7404.813046E-918.607991
CGTGTAC5652.4148067E-618.2786463
GTAGACC5051.8805229E-518.1781043
TAAGGCT9652.1827873E-1117.8366764
CACTGTG5202.4188015E-517.6537367
GTATAGT9151.9826984E-1017.5572681
GTACTAC4000.001498578517.2123931
GTGTATA11350.017.1871191
ACACTGT10104.3655746E-1117.0419736
CCTTAGG4050.00162853716.9998951
CCTATAC6756.978171E-716.9998953
TACACAG9453.1468517E-1016.9998935