FastQCFastQC Report
Mon 11 Jul 2016
MG395078P_S12_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395078P_S12_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968065
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT2804.0025043E-628.7822976
CCCTAGC2550.002220027622.5766831
GAGTGCG2550.00222136122.574359
GTCTTAC6255.620677E-1022.1070881
ACCCGTC3152.935344E-421.9293677
GTGTGTG2750.003442995720.9325797
CCCGTCC3050.006267389718.8736388
GTCTTAA6753.2734533E-818.7637311
GTCCTGG3100.00688003418.5711421
GTCCCTA3100.00688003418.5711421
TAGGACC4351.2056258E-418.5265354
GTATAGA11900.018.383871
GGAGTGC3150.00754978418.2744738
GTCTAGG3800.001038278218.180171
GTATTAG17900.018.0108951
TACACTG8951.382432E-1018.0090335
TGTGTGC4551.701045E-417.7121818
GTATAGG7901.0362783E-817.4897841
GTATTAT19400.017.2118111
CTAGGAC4100.001728887216.8481733