FastQCFastQC Report
Mon 11 Jul 2016
MG395078P_S12_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMG395078P_S12_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968065
Sequences flagged as poor quality0
Sequence length32-241
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAG1600.006309246628.7756469
AGGCCTG1700.00848182927.0829625
TAGACCG5104.3655746E-1127.082965
CTAGACC5802.2191671E-1023.8143274
CGCACCG2901.6794603E-423.8143275
GTATAGA11150.022.7108231
TATAGGG4653.615387E-722.277922
GTATAGT7409.094947E-1221.7761631
TACACCG9650.021.4699135
AGACCGC3304.025599E-420.9277426
CTGTGTG2750.003447611620.9277429
AGGGTGT2800.003827019820.5540336
CTATACA10350.020.017844
TAGACTG4709.8568E-619.5919285
CCCTATA4751.0799586E-519.3856962
TATACAG14400.019.1837645
CCAGTAC8006.002665E-1018.704173
CACGCTA3200.00827830517.984789
CTAGGAC3200.00827830517.984783
CTACACC13450.017.9714074