FastQCFastQC Report
Mon 11 Jul 2016
ME168012C_S11_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameME168012C_S11_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911349
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTGT2351.5414116E-435.621994235
CCCGGGA1350.002741975234.117352
AGAGCCC2404.625562E-528.7865148
GTATAGG6850.028.5795291
TAGAGTG4407.3177944E-926.1695585
AGGGTGT2300.001217319525.0317526
CTGTGCG2951.880854E-423.4195379
GGGCACT2550.00221937122.5776586
GGGTGTA2750.003439920720.9356467
GTCTACG6101.02099875E-820.7663211
GTACTAG6101.02099875E-820.7663211
ACTATAC7603.0195224E-1019.6960353
TGTACAC5901.6370541E-719.5162813
CCTGGAC4751.0763313E-519.393023
TGCGCCG3000.00569163319.191015
AGGGAGT4801.1781069E-519.191016
ATAGGGG3607.217081E-419.1910083
TAGGGGT3657.918664E-418.928124
TATAGGA8557.094059E-1118.8543262
GTATTAG19750.018.6586231