FastQCFastQC Report
Mon 11 Jul 2016
ME168012C_S11_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameME168012C_S11_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911349
Sequences flagged as poor quality0
Sequence length32-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGGGC1300.002281044435.421983
CCTATAC6000.030.699053
TAGGACC4402.3283064E-1028.7803614
GGACCGT3754.5076376E-827.6291476
CTAGGAC3651.0973454E-625.2320983
GTAGGAC6104.1654857E-1022.6468413
TAGACAG7409.094947E-1221.7797365
TCCTATA6101.0248186E-820.7596052
GCCTTAA3354.4503695E-420.6187671
AGGACCG3955.7493155E-520.4012685
GTAATAC8703.6379788E-1219.8485263
GTATACG8254.3655746E-1119.535761
GTATTAG19000.018.7829741
GGGGCAG4500.00664497718.682085235
ATACTGC10700.018.2903236
GGACTCC3150.007552380718.2732456
GTCTAAG5051.8280443E-518.237061
TAGGCTG7707.55972E-917.9410065
TAGGACT6454.1868225E-717.8482864
CTATACT9702.3646862E-1117.8022864