FastQCFastQC Report
Mon 11 Jul 2016
M6739322A_S2_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM6739322A_S2_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634839
Sequences flagged as poor quality0
Sequence length32-241
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC604.728006E-577.692823
TTATATA801.9641378E-458.269612
TTATATT650.00631559453.7873344
TAAGAGT700.00846575449.945384
TAGTACA1057.5148896E-444.3958972
GTATAGG2159.429641E-943.3702621
ATATACA1354.973785E-543.1626784
CCATTAA1300.002145927935.8638731
TCTATAC1300.00214758835.8582233
GTATACC1651.6219304E-435.3149153
GAGTAAC1802.7013803E-432.3771061
TATACCA1802.7038794E-432.3720054
GTATAGA1450.003665185332.1538161
GTCTAGG1550.005079490630.0793761
GAATAGA2055.7878456E-428.428681
GTTCTAT1650.006894262528.2563841
TAGTAGA1650.006894262528.2563841
CCGTATA1650.006899568728.2519322
TATACAG1650.006899568728.2519325
GGTCTAT2505.6198856E-527.9738221