FastQCFastQC Report
Mon 11 Jul 2016
M6739322A_S2_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM6739322A_S2_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634839
Sequences flagged as poor quality0
Sequence length32-241
%GC65

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG1308.922143E-962.6464085
TAGTACC751.4394346E-462.049784
CTACACT903.5435765E-451.7081534
ATACACT700.008522226549.861434
TTGTAAC954.6269584E-448.9866682
TACAGTA954.6269584E-448.9866687
ATATAGC1350.002605780734.47211
TAAGCAT1350.002605780734.47214
CTCTATA1701.9521912E-434.2186282
CGATAAG2650.004092712431.433489235
GACTAGC1903.7494893E-430.6166691
GTACAGA2753.2033222E-629.6146681
TGTATGG1600.005985753629.0858352
TCCAATA1650.006956472528.2044432
TATAGAA1650.006956472528.2044432
TATGGAC1700.00804756627.3749033
GTAGACA2157.7287806E-427.0565914
ATCAGAG1750.00926959926.5927622
GTTCTAT2709.607918E-525.8540741
TCCTGTA3201.0405427E-525.4501042