FastQCFastQC Report
Mon 11 Jul 2016
M6739216A_S1_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM6739216A_S1_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152331
Sequences flagged as poor quality0
Sequence length32-241
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA7450.030.8389591
GTCTAAT1703.681338E-430.7152731
GTATAAG5500.028.4814341
TATAGGA3658.929783E-825.749222
GTACATA3851.4955367E-724.4126591
GTGTAAG3552.0133757E-623.5339551
TGTACTA2702.0016804E-423.2060875
GTATATA5053.5706762E-922.7475471
GTGTAGG4153.0858064E-722.6478861
GTATATG6555.456968E-1222.3213271
TTCTATA5655.4023985E-1022.179272
CTTGGGA2450.003103037421.3117123
ATAGGAG4056.316619E-620.6276323
GTGTACA3555.3530395E-520.5922091
TAGACGT4157.799998E-620.130584
GTTCTAT6251.902663E-920.050931
ACAGTGG6452.8176146E-919.4283528
TAGGAGA3257.000101E-419.2789024
GTATAGG5502.1869891E-718.9876211
TATAAGG6603.748937E-918.98682