FastQCFastQC Report
Mon 11 Jul 2016
M6739216A_S1_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameM6739216A_S1_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152331
Sequences flagged as poor quality0
Sequence length32-241
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGTA2650.001830913237.040977235
TGCTAGA1300.003837181731.8562432
TACTGTG4101.0913936E-1130.302287
ACTGTGT3602.6757334E-928.759118
GTCCTAC2605.137592E-627.8742121
GTCCTAT2655.957738E-627.3482861
CTCTAAG2409.5412855E-525.8831961
GTATAAG5309.094947E-1225.3948361
TACACTG6650.023.353265
ATACTGT7750.022.7104196
TTCCCAA5550.002506097522.107788235
ATAAGGG7550.021.9407223
ACTGTGC6451.382432E-1020.8670758
TATAGGA3505.1295898E-520.7065582
TAAGGGG5551.1719749E-820.5200124
CTATACT4558.128318E-720.4790134
CCTATAC5651.4393663E-820.1568263
TATATAC5751.7609636E-819.8062743
TAGTGTG3206.6824496E-419.41247
CTATACC12300.019.359794