FastQCFastQC Report
Wed 6 Jul 2016
Undetermined_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317915
Sequences flagged as poor quality0
Sequence length50-241
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGA505.520632E-5177.17766235
GAAGGTT500.003934786760.6104774
CTGCACA1103.4481185E-545.9170279
GCCCAAC1150.002371100235.136512
CCCAACG1450.007372152527.8668843
AAAGGAG1450.007372152527.8668842
AAAAAGG3404.8111362E-48.9133051
GAAAAAA23554.591274E-46.0052846
AGAAAAA28508.872229E-45.31670865
AAAAAAG25300.00695916735.19062047
AAAAATA29850.00164325475.0762547