FastQCFastQC Report
Wed 6 Jul 2016
Undetermined_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameUndetermined_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317915
Sequences flagged as poor quality0
Sequence length50-241
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGG100.002047646219.606322
CTATCCG550.004124893359.892634
GCATTCC751.9147273E-458.5616843
ATTGAGC600.0058208854.901582
AGTGGGA750.006781977652.818497225-229
AAATCTC2203.525828E-529.9463165
GAAATCT2355.528494E-528.034854
GCCCAAC2109.433749E-426.143611
CCACCCG3050.0082248418.0005199
CTCGTAT4900.007740359813.4452869
CCGACCC5000.00884593713.1763799
AGAAATC6200.002536245412.3971313
AATCTCG6750.004789672311.3869956
AAAAGAA37950.09.54811
CAGAAAT11300.00235596448.7453852
GAAAAAA40059.094947E-127.40246065
AAAGAAA49753.6379788E-116.40058662
AGAAAAA40302.7243004E-86.2666824
AAGAAAA55151.7362254E-85.3756763