FastQCFastQC Report
Wed 6 Jul 2016
MU572163T_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99241
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCTT1402.983153E-585.03006235
AGAGCAC400.001292471980.21396
TAGACTG550.00456852658.3373839
GCACAAC550.00456852658.3373837
GTACTAG600.00644632653.4759331
CTCTAGA650.00884591749.3623961
TGGACTA905.345282E-447.534165
CTCTTGT1451.2491741E-436.879951
GGTGCCA350.002557620434.59919215-219
ATATGGT1400.004683430330.5576781
GTTAAAG1753.7692973E-430.5576761
TATGGTA1450.00556008829.5039622
GTAGTTG1450.00556008829.5039623
GTATTAG1500.006561689528.52051
ATTAATC1500.006561689528.52053
GGGAGCT300.00658669928.52049630-34
TACAGAA1906.1004865E-428.1452262
CATCAAC1600.00899074226.7379653
ATCCATA2100.001094564425.4647311
ATATAAG2200.001435502524.3072411