FastQCFastQC Report
Wed 6 Jul 2016
MU572163T_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMU572163T_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99241
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCC303.2941418E-4113.138868
ACACTGC550.003656417461.712118
GGACATA904.0505265E-450.2839366
TTATGCC700.0094931148.4880874
GTATCCC700.0094931148.4880879
GTACAGT955.288404E-447.6374176
GTATATA955.288404E-447.6374171
AGTATCG1202.9369106E-547.1411935
GTATATG1304.7118287E-543.5149461
ATCGACC1058.6590217E-443.100528
TAATACA1702.2862953E-433.2761344
CCTTTAA1752.7103868E-432.325391
GTATAAA1752.7103868E-432.325391
ATTATAC1400.00355650432.325393
TATTACA1450.00422311231.2107184
CTAATAC1450.00422311231.2107183
ATTGTAA1853.7545877E-430.5780721
TACAGTA1500.004984921330.1703627
TCTGTCT1550.00585154229.1971238
GTATTGA2800.00420175320.203371