FastQCFastQC Report
Wed 6 Jul 2016
BS20102N_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104779
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGGG650.00810805250.464337
CACAGGG650.00810805250.464337
CTAGGCT1008.059272E-443.7357524
ATGGTGT1350.0035191432.396856
AACCCCC350.00813994127.30959170-174
TATGGTG1650.00938238326.5065175
CCTTTAT3604.1390667E-521.2604351
GTATTAT2650.003724092620.6300721
TAATGGT3508.3888206E-418.7438934