FastQCFastQC Report
Wed 6 Jul 2016
BS20102N_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameBS20102N_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104779
Sequences flagged as poor quality0
Sequence length50-241
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGG150.0062087825151.523326
ACCGAAC400.001015880685.2318658
GAGGCGG400.001015880685.2318656
ATTAGGC600.00507123556.821243
CTCTATA600.00507123556.821242
GTCTAAG852.9893307E-453.4788171
TGTTCGC852.9893307E-453.4788178
TAGGCTC1101.710758E-551.655675
GTATAGG700.00932943348.7039221
CTCTAGG700.00932943348.7039221
ACATAGC1100.001065408741.324548
TTACGAG1150.001325736339.527823
TTAGACA1901.0159487E-535.88714
AACAGCG1300.002420645434.966927
GTATAAA2152.367851E-531.714181
CTATTAG1450.004133353531.349651
ATACTTA1600.00668824128.4106226
TATTAGG1650.007772281827.5496942
GTTCTAA1700.00899063626.7394081
TAGACAT1700.00899063626.7394085