FastQCFastQC Report
Wed 6 Jul 2016
607239_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename607239_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234841
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATAA200.008691206968.2227235
GATATGG1750.001973634221.30804235
TATGGGA450.004680871558.0053942
GCTTTAC500.007101750552.2048571
GTGTATG850.001117719140.9449841
ATACTGC1200.006062366529.0026976
GTACATG1250.007399724327.8425881
GATTACT650.00857551627.019127195-199
TTGTCCG2500.00998698617.4016179
GTGTTGA4350.001013001914.0013031
GGTTGTC4450.001202741813.68666657
GTGCTGG12557.287054E-47.6262071
TGCTGGC12450.0056786576.98860172