FastQCFastQC Report
Wed 6 Jul 2016
607239_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename607239_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234841
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTAAA50.009622691921.9005235
TGATTAA200.0077443235140.75327200-204
TAGTCTG304.692166E-4103.556759
AACAATA358.6593674E-488.7629242
GTAAGTG550.005199602356.48559
TAGGGTC1000.001055724641.42274
ATCCATG1150.002098050740.08263235
ATGACAG1400.005498761329.5876435
ATCTTTG1450.0065271728.5673797
GGACTGC3450.001102587418.009876
TCGGGGG4100.003495199615.1546463
GACTGCG4150.003788784114.9720617
CTACACC4200.004102858714.7938224
TGAGCGG4350.005179673414.28368955
CCGTGCT5350.001297742613.5494819
ACCGTGC7657.392304E-613.5368318
GAACGGG4800.00993529712.9445936
CCTGCAC6150.003696367811.7869473
TGGACCG7057.6500577E-411.7511215
TGCACCG7300.001024498811.3486845