FastQCFastQC Report
Wed 6 Jul 2016
606111_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165197
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGA100.00138856052417.9124235
GATTCTA100.00138856052417.9124235
TTAGTCA200.00555273751208.9562235
AAGCACT700.0036761633180.21706230-234
TAGTGCA450.005518574755.634674
GAGTAGA500.00837108450.0712051
TCCCTGT500.00837108450.0712057
TGACTCA500.00837108450.0712056
TTACGCC700.00977373448.138668225-229
TACTGGG1100.00485450139.901855220-224
TCTACCG1050.003866216431.7912465
TTGAACT1100.00485450130.3461884
TTGGGTG1250.00906270326.7046434
TTTTTTT509.075926E-526.0716125-129
GCCCAAG2301.0509102E-525.3984411
GTACTGG2101.6574706E-423.8434351
TGGACAG1950.003020342421.3979515
GTAGGCG3150.00165434986.939366230-234