FastQCFastQC Report
Wed 6 Jul 2016
606111_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename606111_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165197
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAACT100.0021878881214.768084
ATAGAAC400.001273451380.538033
GTATAGC607.07625E-571.589361
CCATACT600.006351763453.692024
TGCTACA600.006351763453.692022
TATGTTG600.006351763453.692025
ACATAGA650.00871625549.5618671
GTTCTAC1351.4366578E-647.7262421
GTGCTAC1409.950753E-538.3514481
GTGCATA1150.001754136837.350971
TAGACCG1600.00883236526.846015
GTCCAGG4850.00835817613.2846241