FastQCFastQC Report
Wed 6 Jul 2016
506776_reverse_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_reverse_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206296
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACTTC450.003556073262.1711546
ACGTTTT550.00786785150.8673066
TCATTTG550.00786785150.8673065
TTTTGCA550.00786785150.8673069
ATCTAGC450.007449520840.399498230-234
CTCCAGA1100.0028194933.9115371
GTCTTGA1604.9942394E-429.1427271
AGGACTG1400.00916760926.6447815
TGGACTG2107.8029225E-526.644785
ACCGCTT3501.1395946E-418.6513468
GGACTGC2650.00936578617.595616
GTAGACC2650.00936578617.595614
GGGTGTG4655.4549284E-516.0441687
GTGCTGT3950.00546999414.1655791
GCCCCAG4150.007586786513.4829011
ATGTACG1150.00395239911.972363210-214
ACCGCCC7200.00218618810.3618598
GCACCGC14550.00167647137.0503376