FastQCFastQC Report
Wed 6 Jul 2016
506776_forward_paired.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename506776_forward_paired.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206296
Sequences flagged as poor quality0
Sequence length50-241
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATA100.0023078206210.985213
ACATATG450.002181439470.32841
TAATGGT500.003312305363.2955674
AGACTAC350.001936709336.62167225-229
TTGAGGA1552.0119692E-434.0298733
ATATGCC1300.003493916232.4592633
GTCTACA1400.00502134330.1407432
TCTACAC1400.00502134330.1407433
GTCCTAC1450.005960899429.101411
TCCAGTG2100.001190219925.1172872
GACAGTG2200.00156076323.9755927
TCAAGGT3054.244832E-420.7526423
GTCTTGC2950.00853929517.8801021
GTTGAGG4150.003347573315.2519442
CTGGACA4500.005732808314.06568054
GACAGCG6903.5416946E-513.7599067
GTGCAGG5500.001389256713.42633151
ACAGCGC6600.0054490211.1886098
AGTCCAC1950.0096921918.65580410-14